TEDE Coleção:http://www.bdtd.uerj.br/handle/1/35342024-03-29T10:35:22Z2024-03-29T10:35:22ZDistribuição de proteoglicanos e outros componentes da matriz extracelular em artérias normais de adultos jovensBeltré, Raquel Libanesa Rosariohttp://www.bdtd.uerj.br/handle/1/208812024-02-26T18:24:05Z2019-02-18T00:00:00ZTítulo: Distribuição de proteoglicanos e outros componentes da matriz extracelular em artérias normais de adultos jovens
Autor: Beltré, Raquel Libanesa Rosario
Primeiro orientador: Cardoso, Luiz Eduardo de Macedo
Abstract: Although cardiovascular diseases are the main cause death globally, factors affecting the differential location of atherosclerotic lesions are still not well understood. Of the arterial extracellular matrix components, proteoglycans are thought to be involved in atherogenesis by binding plasma lipoproteins. We thus investigated whether the distribution of proteoglycans and other proteins in normal human arteries correlates with the susceptibility of a given artery to atherosclerosis. Arterial samples from the left coronary, abdominal aorta, common iliac, right renal, and pulmonary trunk were obtained during autopsy of 15 males aged 17-28 years who had died of accidents. Morphometry of arterial layers was done on tissue sections stained with hematoxylin-eosin, Masson's trichrome, and Weigert's resorcinfuchsin, while immunohistochemistry was carried out using primary antibodies against the proteoglycans decorin, lumican, biglycan, and versican (v1), as well as against fibronectin and smooth muscle alpha-actin. Total collagen concentration in the arterial wall was determined by a biochemical method, and the intensity of immunostaining was assessed by a color deconvolution technique. Data were analyzed by one-way ANOVA followed by the Holm-Sidak multiple comparisons test.
Protein expression of proteoglycans did not differ among the arteries, but varied significantly between layers. In all arteries, decorin was expressed mostly in the adventitia. The intima/media ratio was much higher in atherosclerosis-prone arteries (coronary, aorta) as compared to atherosclerosis-resistant vessels (renal, pulmonary trunk). Collagen content was similar in all arteries. Arteries at a higher risk for
atherosclerosis consistently have thicker intimas, whereas their proteoglycan contents do not differ from those of vessels at a lower risk.
Instituição: Universidade do Estado do Rio de Janeiro
Tipo do documento: Dissertação2019-02-18T00:00:00ZAlterações no tecido esplênico e perfil da resposta imunológica de camundongos esquistossomóticos submetidos à ingestão de etanolRosa, Aline Aparecida dahttp://www.bdtd.uerj.br/handle/1/208492024-02-26T18:24:01Z2019-02-26T00:00:00ZTítulo: Alterações no tecido esplênico e perfil da resposta imunológica de camundongos esquistossomóticos submetidos à ingestão de etanol
Autor: Rosa, Aline Aparecida da
Primeiro orientador: Neves, Renata Heisler
Abstract: In schistosomiasis mansoni infection, the spleen is one of the affected organs, causing the increase of its volume (splenomegaly). Ingestion of ethanol through alcoholic beverages can cause spleen atrophy and interfere with immune activity. To gain insight of this association on the spleen and in the immunological response profile, male mice were used as the experimental model. One group of these animals (n = 5) were neither infected nor received ethanol (Group C), another group (n = 5) was not infected, but received orally (gavage) 200 μL / day of ethanol 18% for 28 consecutive days (CE Group). The remaining animals were infected with 80 cercariae (BH strain) of S. mansoni, with one group (n = 7) being maintained the infection only (Group I), and in the other group (n = 7) was administered ethanol (28 consecutive days) beginning on the fifth week of infection, going to the end of the experiment (IE group). At the ninth week of infection, at the end of the gavage, all animals were euthanized and the content of the peritoneal lavage (in RPMI medium) was collected to obtain cytokines produced by peritoneal cells. The spleen was removed, cleaved longitudinally into two parts. One of these parts was fixed and submitted to routine histological processing and histological sections stained in H&E and Gomori reticulin. The other fragment was macerated (in laminar flow) and the cell suspension, after adjustment of cell concentration (2x106), was plated to obtain cytokines produced by lymphocytes. Biochemical and blood analyzes (leukogram) were also performed. Spleen alterations were evaluated through histopathological and stereological analysis (test system D36), white pulp morphometry and megakaryocyte quantification. Cytokines produced by peritoneal cells and lymphocytes were measured by flow cytometry (Citometric Bead Array). The results of the biochemical analysis showed a significant increase of glucose (+ 71%; p=0.0079), cholesterol (+ 22%; p= 0.0079), HDL (+ 27%; p= 0.0079) and albumin (+ 30%; p= 0.0079) in the IE group in relation to group I. The analyzes in the splenic tissue of the IE group showed an increase in the amount of trabeculae and megakaryocytes, decrease in reticular fibers, as well as organizational changes in white pulp and red pulp. The IL-6 cytokine produced by the peritoneal cells presented a significant increase (+32200; p = 0.0018) in the IE group when compared to the C group. Changes in this study demonstrate that ethanol ingestion interferes in some experimental parameters of schistosome infection, such as changes in spleen tissue and in the pattern of cytokine production.
Instituição: Universidade do Estado do Rio de Janeiro
Tipo do documento: Dissertação2019-02-26T00:00:00ZAlterações no tecido esplênico e perfil da resposta imunológica de camundongos esquistossomóticos submetidos à ingestão de etanolRosa, Aline Aparecida dahttp://www.bdtd.uerj.br/handle/1/208402024-03-04T02:06:15Z2019-02-26T00:00:00ZTítulo: Alterações no tecido esplênico e perfil da resposta imunológica de camundongos esquistossomóticos submetidos à ingestão de etanol
Autor: Rosa, Aline Aparecida da
Primeiro orientador: Heisler Neves, Renata
Abstract: In schistosomiasis mansoni infection, the spleen is one of the affected organs, causing the increase of its volume (splenomegaly). Ingestion of ethanol through alcoholic beverages can cause spleen atrophy and interfere with immune activity. To gain insight of this association on the spleen and in the immunological response profile, male mice were used as the experimental model. One group of these animals (n = 5) were neither infected nor received ethanol (Group C), another group (n = 5) was not infected, but received orally (gavage) 200 μL / day of ethanol 18% for 28 consecutive days (CE Group). The remaining animals were infected with 80 cercariae (BH strain) of S. mansoni, with one group (n = 7) being maintained the infection only (Group I), and in the other group (n = 7) was administered ethanol (28 consecutive days) beginning on the fifth week of infection, going to the end of the experiment (IE group). At the ninth week of infection, at the end of the gavage, all animals were euthanized and the content of the peritoneal lavage (in RPMI medium) was collected to obtain cytokines produced by peritoneal cells. The spleen was removed, cleaved longitudinally into two parts. One of these parts was fixed and submitted to routine histological processing and histological sections stained in H&E and Gomori reticulin. The other fragment was macerated (in laminar flow) and the cell suspension, after adjustment of cell concentration (2x106), was plated to obtain cytokines produced by lymphocytes. Biochemical and blood analyzes (leukogram) were also performed. Spleen alterations were evaluated through histopathological and stereological analysis (test system D36), white pulp morphometry and megakaryocyte quantification. Cytokines produced by peritoneal cells and lymphocytes were measured by flow cytometry (Citometric Bead Array). The results of the biochemical analysis showed a significant increase of glucose (+ 71%; p=0.0079), cholesterol (+ 22%; p= 0.0079), HDL (+ 27%; p= 0.0079) and albumin (+ 30%; p= 0.0079) in the IE group in relation to group I. The analyzes in the splenic tissue of the IE group showed an increase in the amount of trabeculae and megakaryocytes, decrease in reticular fibers, as well as organizational changes in white pulp and red pulp. The IL-6 cytokine produced by the peritoneal cells presented a significant increase (+32200; p = 0.0018) in the IE group when compared to the C group. Changes in this study demonstrate that ethanol ingestion interferes in some experimental parameters of schistosome infection, such as changes in spleen tissue and in the pattern of cytokine production.
Instituição: Universidade do Estado do Rio de Janeiro
Tipo do documento: Dissertação2019-02-26T00:00:00ZConsequências da dieta rica em frutose parental, ou só materna, ou só paterna no metabolismo hepático da prole adulta de machosCarapeto, Priscila Vianahttp://www.bdtd.uerj.br/handle/1/203572024-02-26T18:24:05Z2018-02-28T00:00:00ZTítulo: Consequências da dieta rica em frutose parental, ou só materna, ou só paterna no metabolismo hepático da prole adulta de machos
Autor: Carapeto, Priscila Viana
Primeiro orientador: Mandarim de Lacerda, Márcia Barbosa Águila
Abstract: The study aimed to evaluate the consequences of feeding high-fructose diet (HFR) to mother or father, and to both mother and father, on progeny. Parents received HFR or control diet (C) for eight weeks before mating until weaning. After weaning, pups (identified first by the mother’s diet, then by the father’s diet) only received C diet until three months of age. Parents: blood pressure (BP) was higher in HFR parents, compared to their controls (father +27%, mother +35%, P < 0.0001). The HFR parents, had an enlarged liver (father +15%, mother +18%, P = 0.0290), greater hepatic triacylglycerol content (father +89%, mother +41%, P < 0.0001), hypercholesterolemia (father +25%, mother +24%, P = 0.0158), hypertriglyceridemia (father +45%, mother +39%, P < 0.001), higher plasmatic leptin and lower adiponectin (Leptin: father +68%, mother +55%, P < 0.0001; Adiponectin: father -6%, P = 0.018, mother -21%, P < 0.0001). Offspring: compared to C/C, HFR/HFR had high BP; C/HFR, HFR/C, and HFR/HFR showed higher uric acid and leptin, and lower adiponectin. HFR/HFR showed higher liver inflammation markers (NF?-B, SOCS3, JNK, TNF-alpha, IL1-beta, and IL-6). HFR/HFR showed an increase of both SREBPB1c and FAS. In conclusion, the study can demonstrate that both mother and father, individually, or in combination fed a HFR diet may affect the metabolism and liver in the adult male progeny. The combination of mother and father HFR enhanced the adverse effects on the offspring health and had other consequences in the offspring programming than the mother and father alone.
Instituição: Universidade do Estado do Rio de Janeiro
Tipo do documento: Dissertação2018-02-28T00:00:00Z