Biotecnologia aplicada à remoção do brometo de etídio por ficorremediação

Abstract

The chemical residues generated by research and/or teaching laboratories at universities and research centers raise a serious concern due to risks posed to the environment if not handled properly. Residues of some organic compound are discarded incorrectly because of inappropriate glass washing and equipment cleaning. Ethidium Bromide (EtBr) is an organic compound widely used as a DNA intercalator in molecular biology procedures and the EtBr ability of intercalating itself in the DNA molecule, makes it a toxic substance. Among biological wastewater treatment strategies, the use of microalgae (phycoremediation) has been appreciated as a potentially effective option to remove target contaminants from water. The objective of this study was to evaluate the ecotoxicological effect of EtBr and its removal in water by three species of unicellular microalgae individually (Chlorella vulgaris, Desmodesmus subspicatus, and Raphidocelis subcapitata) and in a mixture (Mix).EtBr concentration that caused effect in 50% (EC50) of the microalgae D. subspicatus was 0.375 mg/L, and the highest concentration that did not cause effect (NOEC) was 0.25 mg/L.In the first treatability assay of 24h, the R. subcapitata when exposed to 2.0 mg/L of EtBr removed 34% including photodegradation + microalgae (absorption and degradation), being 13% by photodegradation and 21% by microalgae itself, and 13% of 21% occurred within the first 6 hours.In the second treatability assay, no large variations in the number of algae/mL were observed after 3 hours of exposure to a 0.5 mg/L solution of EtBr in mineral water, starting and ending with 106 algae/mL, excepting for R. subcapitata that ended with 107 algae/mL. The results in absolute values of EtBr removal (measured by fluorescence) after 1 h of treatment were: Mix>D. subspicatus>C. vulgaris>R. subcapitata. After 3 h the best results in absolute values (mg of EtBr) were: Mix>D. subspicatus>R. subcapitata>C. vulgaris. However, if relative microalgae density (mg/algae/mL) data are considered, the ranking after one hour was: C. vulgaris> Mix>D. subspicatus>R. subcapitata; after 3 h the ranking was: D. subspicatus>C. vulgaris> Mix>R. subcapitata. Therefore, it was concluded that, despite the low percentage of EtBr removal (<11%) achieved with Mix, this was due to the low microalgae density 106 algae/mL in the treatment assays. Based on these results, it was possible to estimate the density of each microalgae species required for the total EtBr removal as following: 1010 algae/mL for C. vulgaris, D. subspicatus and Mix; and 1011 algae/mL for R. subcapitata.