Desenvolvimento de metodologia para caracterização de hidroxiapatita pelas técnicas de MEV, MET e DRX

Abstract

Teeth and bones contain about 30% to 70% hydroxyapatite (HA) and are of great importance in the vertebrate calcium and phosphorus reserve. HA is considered the most stable phase in a physiological environment and has biocompatibility and bioactivity properties. In this way, it helps in bone growth where osteoconductivity is located, establishing the chemical links between it and bone tissue (bioactivity) and the proliferation of bone cells (osteoblasts, fibroblasts and others). The HA was obtained from precipitation reaction between Ca(OH)2 and phosphoric acid, H3PO4, where NH4OH was used for pH correction. The products used, except for Ca(OH)2, were of analytical grade and used in the additional treatment. In order to obtain HA through chicken egg shells as a source of calcium ions, considering that eggshell is a product that is discarded, seeking to reuse it, several ways of harnessing it have been developed. of these, is the obtaining of HA, techniques such as X-rays, Scanning Electron Microscopy and Transmission Electron Microscopy were used to characterize the material. The objective of this work was the development of sample preparation techniques for the generation of good resolution images in the scanning electron microscope and high resolution images in the transmission electron microscope, with the characterization methodology developed, varying the ultrasound time. , the drying time, establishing specific stub preparation standards, in order to obtain satisfactory results, which allowed us to characterize the HA.