Modulação por Sucupira (Pterodon polygalaeflorus, Benth) da distribuição de Linfócitos T ativados e T de memória em linfonodos (LN) de camundongos com Artrite Induzida por Colágeno tipo II (CIA) e da mielopoiese em baços de camundongos primados com Adjuvante Completo de Freund (AFC)

Abstract

Seeds of the Pterodon gender trees, mainly knowed as Sucupira, have been used by folk medicine to treat rheumatoid diseases. The present study aims: to study the possible action of hydroalcoholic extract of Pterodon polygalaeflorus (EHPp) seeds in the treatment of Collagen Induced Arthritis (CIA) in DBA/1J mice. It was verified the Lymphocyte memory phenotype distribution in animals lymphonods (LNs) with CIA; a possible EHPp treatment interference on the second increase of splenic mieloid cells (Mac-1+) caused by CII booster for CIA induction and it was confirmed the granulocyte phenotype of those mieloids cells. Besides, with another experimental model with 10 days duration, we studied the myeloid cells Mac-1+: if the EHPp treatment interferences in splenic myelopoiese induced by AFC injection in DBA/1J mice and to evaluate casual difference between the activation degree of those cells in animals AFC primed treated or not with EHPp. The CIA was induced in DBA/1J male mice, with intradermally (i.d.) injection of CII in AFC with a CII booster intraperitoneally (i.p.) three weeks after the first injection. The daily oral treatment with EHPp, during 30 days, started on the CII booster day. In the short duration model, with the intention to verify the possible increase of Mac-1+ cells in the spleen, the animals were orally treated with EHPp, once a day, during 9 days, and the AFC i.d. injection performed in the 5th day of treatment and the animals were sacrified on the 10th day. The results were: the EHPp treatment interferes on Lymphocyte T distribution with memory phenotype in animals with CIA; preliminar results indicate that EHPp treatment does not interfere on the second increase of splenic mieloid cells caused by CII booster injection to induce CIA; mieloid cells in the spleen of health animals or in animals injected with AFC have granulocitic phenotype (GR.1+); the EHPp treatment reduced the splenic mielopoiese induced by AFC injection; significant fraction of Mac-1+ cells in spleen of primed AFC animals treated with EHPp had an increased production of the oxigen reactive species in relation of health animals and EHPp treated animals. Together, those results indicate a possible anti-inflamatory action of EHPp because it prevented inflamatory cells increase in the spleen of AFC primed animals, and also an imunosupressive activity, since it reduced Lymphocyte recently activated and memory Lymphocytes in the LNs of CIA animals related to CIA animals. It suggests a possible EHPp action in activation and migration Lymphocytes processes.