Efeito bactericida e alteração de cor proporcionada por medicações antibióticas em diferentes consistências

Abstract

The aim of this study was to analyze the effect of triple antibiotic medication in pasty and solid consistency against of E.faecalis biofilm formed in human dentin and measure by using spectrophotometer if there was any change in the coronary color of the dental elements. Seventy teeth were divided into two control groups (n=5) and six antibiotic groups (n=10) according to the formulation and consistency of the antibiotic medication, as followed: CN – negative control, with destilled water applied, CP – positive control, Group 3MIX-sol – triple antibiotic paste in solid consistency, Group 3MIX-past – triple antibiotic paste, pasty consistency, Group 2MIX-sol – triple antibiotic medication in solid consistency, Group 3MIX-mod-sol – triple antibiotic medication modifed with clindamicyn, solid consistency, Group 3MIX-mod-past – triple antibiotic medication modified with clindamicyn, paste consistency. The coronary color change was measured by a spectrophotometer according the CIELAB system, before and three weeks later the antibiotic medication was used, the ΔL*, Δa*e Δb* were collected to verify if it had luminosity, axis green-red and blue-yellow changes. To analyze the antibacterial activity of the medications tested, the specimens were contaminated with E.faecalis culture, for 30 days, aiming the growth of intra radicular biofilm. After that, the specimens of all groups were irrigated with sodium hypochlorite 1,5% and EDTA 17% by five minutes each, so the root canals were dried and the antibiotic paste could be applied, remaining in the dental element for 15 days. After 15 days the antibiotic paste were removed from the root canals by irrigation with destilled water and EDTA 17% and the samples were placed into test tubes filled wtih Entereccocosel medium to analyze the descontamination of them in 48 hours at the incubator. The NC group went through the same process of decontamination, but had its root canal filled with distilled water and the PC group only went through external surface decontamination. All the groups with minocycline in formulation had coronary color change (P < 0,05), while the staining of the other groups, including control, remained stable (P > 0,05). The NC and the 3MIX-mod-paste group was the only experimental group that showed bacterial growth after terapy (P < 0,05). The 3MIX-sol showed less coronary color change compared to 3MIX-past. The 2MIX-sol, 2MIX-past, 3MIX-mod-sol and 3MIX-mod-past groups did not show change in dental color. The antibacterial effect of the 3MIX-sol, 3MIX-past, 2MIX-sol and 2MIX-past groups showed to be efficient agains E. faecalis biofilm. The 3MIX-mod-past group allowed bacterial growth in the Enteroccocosel medium, which did not occur in the 3MIX-mod-sol group