Efeito do transplante de células de medula óssea no perfil inflamatório e resistência à insulina em tecido adiposo de camundongos hiperalimentados

Abstract

Obesity is a disease that grows up in the worldwide. For that, it came up a big deal to control that disease. It can be determined through the increase of abnormal adiposity and It has in consequence several types of metabolic changes. The alterations involved the production of pro-inflammatory cytokines, such as TNF-a. that impairs the insulin pathway in peripheral tissues. For that, cell therapy using Bone Marrow Cells (BMC) emerges as one of the promising alternatives in the treatment of metabolic diseases such as insulin resistance and type 2 diabetes. The bone marrow is composed of a stem cell population that secretes through paracrine way immunoregulator factors, growth factors responsible for tissue repair. However, stills unclear about the intracellular mechanism that regulates the BMC potential in the improvement of the glycemic, insulinemic index through the insulin pathway. For that, we induced the overfed mice to obesity model. We evaluated the glycemia, insulinemia, lipidic profile, immunostaining for TNF-a and expression of proteins related to insulin pathway, responsible for the activation of Glucose Transporter 4 (GLUT4) in white adipose tissue (WAT). Our results indicate that CMO therapy promoted improvements in glycemic indexes, hyperinsulinemia, triglycerides, TNF-a immunostating and GLUT4 content. Total cholesterol levels were also apparently reduced although it did not significant statistically. Thus, we suggest that CMO therapy promoted greater glucose uptake and improved inflammation in WAT, which reinforces research in favor of CMO therapy for patients with metabolic diseases.