Avaliação do efeito de lasers monocromáticos e dicromáticos de baixa potência em culturas de Pantoea agglomerans

Abstract

Despite the beneficial effects of low power lasers on wound healing, their application for treatment of infected injuries is controversial because low power lasers could stimulate bacterial growth exacerbating the infectious process. Thus, the aim of this work was to evaluate in vitro effects of low power lasers on survival, morphology, cell aggregation, resistance antibiotic, division rate and biofilm formation of Pantoea agglomerans. P. agglomerans samples were isolated from human pressure injuries and cultures were exposed to low power monochromatic and simultaneous dichromatic laser radiation. Fluence, wavelength and emission mode were those used in therapeutic protocols for wound healing. Data show no changes in areas of colonies, morphology and cell aggregation, but dichromatic laser radiation decreased biofilm formation and bacterial survival in exponential growth phase and monochromatic red and infrared lasers increased bacterial survival at the same fluence. Monochromatic red laser at low dose increased biofilm formation and infrared at high dose decreased resistance antibiotic to ampicilin. Low Power Lasers modulate resistance antibiotic and biofilm formation of Pantoea agglomerans, but these depend on laser irradiation parameters, since dichromatic laser radiation induces biological effects that differ from those induced by monochromatic laser radiation. Thus, simultaneous dichromatic low power red and infrared lasers could be a new option for treatment of infected wounds, reducing biofilm formation, no altering antibiotic resistance and division rate in Pantoea agglomerans cultures.