Atividade da dipeptidil peptidase 4 (DPP4) e sua associação com biomarcadores inflamatórios e reatividade microvascular em indivíduos com excesso de peso e sem diabetes

Abstract

In this cross-sectional study, the association between constitutive dipeptidyl peptidase 4 (DPP4) activity, inflammatory biomarkers, and microvascular reactivity in subjects with excess body weight without diabetes were investigated. Forty subjects withbody mass index (BMI) ≥25.0 kg/m² and absence of diabetes were evaluated. Microvascular blood flow and vasomotion were assessed by light amplification by stimulated emission of radiation (LASER) Doppler flowmetry. The following variables were measured at baseline, 30 and 60 min after a standardized meal: DPP4 activity, blood glucose, ultra-sensitive C-reactive protein, tumor necrosis factor alpha, interleukin-6 (IL-6), plasminogen activator inhibitor-1 (PAI-1), intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 (VCAM-1). DPP4 activity was inversely correlated to VCAM-1 at baseline (P<0.05) and DPP4 activityAUC was inversely correlated with the myogenic componentAUC of vasomotion (P<0.05). In multiple regression analysis, homeostasis model assessment-adiponectin (HOMA-AD), IL-6, VCAM-1, PAI-1, blood flow, and vasomotion influenced DPP4 activity and explained almost 40% of the variance on it. When HOMA-AD, VCAM-1, and blood flow were respectively placed as dependent variables, DPP4 activity exerted a significant effect in all of them. In conclusion, constitutive DPP4 activity was associated with early markers of endothelial proinflammatory activation and microvascular function and may have an influence or even be influenced by inflammation and microvascular blood flow in subjects with excess body weight without diabetes.