Alelos HLA em uma população multiétnica com doença de Behçet com e sem manifestações oftalmológicas

Abstract

The aim of this study was to analyze the HLA class I and II alleles of Behcet’s disease (BD) patients and correlate it with ophthalmic manifestations (OM) in a multiethnic Brazilian population. Methods: A cross-sectional study paired with a control group was used as methodology that included 72 patients with BD with and without OM. Ophthalmology evaluation performed patients with BD through best corrected visual acuity, biomicroscopy and fundus examination compared to 144 matched healthy controls. Fluorescein angiography was also performed in those BD patients with OM. HLA typing was performed using the PCR-SSO technique to evaluate antigens of class I (A, B and C) and II (DRB1, DQB1 and DQA1). It was found that the HLA-B*51 and A*26 alleles were more frequent in BD when compared to controls (23.6 vs 14.6% and 12.5 vs 4.3%, respectively), but did not reveal a greater risk for the development of OM. Of the BD patients, 42 (58%) had OM and 30 (42%) did not. The HLA alleles of BD patients that differentiated those with and without OM were HLA-B*15 (40.5 vs 20.7%, OR=2.59, p=0.0059), HLA-C*02 (33.3 vs 13.4%, OR=3.20, p=0.0024) and HLA-DQB1*03 (64.3% vs 45.7%, p=0.017), whereas HLA-A*03 (0.0% vs 13.3%, p=0.006) and HLA-DRB1*15 (4.8 vs 19.5%, OR=0.21, p=0.0121) were protective for OM. It was conclude, in this first study carried out with the Brazilian multiethnic population, that the alleles found on both groups of BD patients with and without OM were the same. We described as at risk for OM, the HLA-B*15, -C*02 and -DQB1*03 and as protective were A*03 and DRB1*15