Estudo dos efeitos na microcirculação cutânea do uso do gás carbônico tópico medicinal

Abstract

The literature is vast in showing science's quest to increase the viability of randomized flaps in experimental models with animal samples. Several methods using pharmacological agents and physical methods were proposed, but they lacked a uniformity of research that would provide a real comparison of their results, which for the most part do not present scientific significance. The controlled infusion technique of medicinal CO2 (known as Carboxytherapy) proposes the tissue injection of a gas that can be applied locally, is low cost, and has an effective action on the microcirculation. This study was carried out based on the controlled pre-surgical infusion of medicinal CO2 to enhance regional microcirculation and increase the viability of randomized skin flaps on the back of hamsters. Eighty-nine hamters underwent medical CO2 injection prior to the creation of a 5 x 1.5 cm randomized cranial pedicle dorsum flap. In five groups (20ml/min, 40ml/min, 60 ml/min, 80 ml/min and 150 ml/min) we proposed to outline the best infusion speed, we also proposed to define the appropriate number of pre-surgical application sessions (one, four and eight sessions), the infusion plan (subcutaneous and intradermal), the need for heating (heated or not); in addition to comparing the use of CO2 with that of Nitrogen (inert gas). Results: a reduction in the percentage of average necrosis was obtained from 50.76% in the control group to 1.86% in the group treated with CO2 at 60 ml/min, 4 sessions of pre-surgical, intradermal infusion with heated gas. There was gene expression in the RT-PCR for HIF-1 alpha and VEGF in the first infusion session.