Análise comparativa do corpo cavernoso de ratos Wistar submetidos a eutanásia com associação cetamina/xilazina ou isoflurano

Abstract

To evaluate quantitatively and qualitatively the histomorphology of the penis of adult Wistar rats submitted to euthanasia with ketamine and xylazine or isoflurane. Fourteen 16 weeks old male Wistar rats were created and kept under normal conditions with commercial food and water ad libitum. The animals were divided into 2 groups: ISO - animals killed under inhalation anesthesia with isoflurane, and K+X - animals killed under anesthesia with 10% ketamine hydrochloride (150 mg / kg) associated with 2% xylazine hydrochloride (15 mg / kg). Immediately after the animals' death, the penises were dissected and collected, fixed in a 4% buffered formalin solution, and processed for histomorphometric analysis. The surface density of structures of the corpora cavernosa, total area of the penis, the area of the corpora cavernosa with the tunica albuginea, the area of the corpora cavernosa without the tunica albuginea, the area of the tunica albuginea, were evaluated using the software Image J. Statistical analyzes were performed using the un paired Student's t test to compare the data, considering it statistically significant when p <0.05. Regarding the density of connective tissue, smooth muscle and sinusoidal space and the corpus cavernosum areas, there were no differences between animals in the two groups. On the other hand, animals killed with the association of ketamine and xylazine showed a 24.8% higher density of elastic fibers, in relation to animals euthanized with Isoflurane. The euthanasia method affected one of the morphological parameters of the rat penis. The choice of method should be standardized to reduce bias and for results to be considered and reproducible