Expressão proteica de proteoglicanos e outros componentes da matriz extracelular no estroma das diferentes regiões da próstata humana normal

Abstract

Benign prostatic hyperplasia (BPH) and prostate cancer (CaP) are considered some of the most common pathologies in adult men. The particular incidence of these diseases in specific regions of the prostate leads to the conjecture of the existence of heterogeneity in the areas proposed by McNeal. This study aimed to analyze the stromal composition from this different zones, focusing on proteoglycans; for this, normal prostate samples were collected from young adults aged 17 to 28 years old, as they were sectioned for histological analysis and total collagen biochemistry. Primary antibodies against α-actin, decorin, biglycan, versican V1 and fibronectin were used. Immunohistochemistry assay was evaluated from the color deconvolution method and as means between how zones were compared one-way ANOVA pathway following the Holm-Sidak method, the results were considered significant when p < 0.05. The studied material comprised a homogeneous sample of young adults. The biochemical assay of hydroxyproline varied significantly between the zones of the prostate (p < 0.001). The content of fibronectin, biglycan and decorin also showed statistically distinct variations in the stroma of each zone (p < 0.001, p = 0.003, p < 0.001 respectively). The versican V1, although not varying in density by region (p = 0.357), was characterized by its marking almost exclusively on nerves. The α-actin marking also did not vary in quantity and intensity (p = 0.404). The study shows strong evidence of the existence of heterogeneities between different zones of the normal human prostate, which may be associated with different protective or pathophysiological mechanisms.