Estudo da interação de diferentes bactérias na indústria do petróleo e seus metabólitos: monitoramento por microbiologia clássica e biologia molecular

Abstract

The biogenic production of sulfide is one of the main problems of the oil and gas industry, causing corrosion in storage tanks and pipes. This is possible by injecting seawater during secondary oil recovery. In the present work, high levels of sulfate-reducing bacteria and acid-producing bacteria were detected in water / oil samples from various locations in the oil industry, as well as several other microbial groups. 35 samples were analyzed in a preliminary phase and in a second moment another 13 samples, where a wider range of microbial cells was detected. The water and oil samples that showed the highest microbial growth were submitted to metagenomic analysis, which confirmed the presence of a diversity of microorganisms, indicating the complexity of the consortium in the production of sulfide, based on the activity of acid-producing cells and associated species. It was also identified, through chromatographic analyzes, formic acid as a metabolite for the growth of acid-producing bacteria that will serve as a substrate for sulfate-reducing bacteria, thus creating a corrosion- friendly microbiome.