A deficiência materna de vitamina D leva a alterações na morfologia da ilhota pancreática e na cascata de sinalização da insulina na prole de camundongos C57BL/6 adultos

Abstract

To investigate the effects of maternal vitamin D deficiency in glucose metabolism, pancreatic morphology and insulin signaling pathway. We studied the C57BL/6 offspring of from mothers fed one of two diets: SC (standard chow) or VitD- (vitamin-D-deficient). We evaluated the biometry, biochemistry, pancreatic morphology and the expression of proteins in the pancreatic islets. The VitD- mice had increased body weight from birth until the 5th week of life when compared with mice SC. The VitD- offspring showed greater area under the curve of the oral glucose tolerance test, hyperglycemia, followed by reduced the pancreatic beta-cells and the islet masses, then showed hypoinsulinemia. Furthermore, VitD- mice demonstrate a reduction of components of the insulin signaling pathway, IRS-1, PI3-K, PDK-1, AKT and increased FoxO1 as possibly reduced the expression of PDX-1, likely responsible for pancreatic alterations. Along with this, the expression of GLUT-2 and GLK was reduced. These results provide compelling evidence that maternal vitamin D deficiency affects the development of the offspring of the pancreas causing metabolic changes in glucose metabolism, structural changes and insulin cascade.