Efeitos intergeracionais da obesidade materna sobre o pâncreas endócrino de camundongos C57BL/6

Abstract

To evaluate the effects of maternal obesity upon pancreas structure and carbohydrate metabolism in early adult life, focusing on F1 and F2 generations of F0 dams fed high-fat diet during the pre-pregnancy, gestation, and lactation periods. Methods: C57BL/6 female mice (F0) were fed standard chow (SC) or HF diet for 8 weeks prior to mating and during gestation and lactation to provide F1 generation (F1-SC and F1-HF). At 3 months of age, F1 females were mated to produce F2 generation (F2-SC and F2-HF). Male offspring from all groups were evaluated at 3 months of age. Results: F1-HF offspring had significantly higher body mass and glycemia than F1-SC offspring. Conversely, F2-HF and F2-SC offspring had similar body masses. F1-HF and F2-HF offspring showed increased energy intake, glucose intolerance, hyperinsulinemia, hyperleptinemia, hypoadiponectinemia, insulin resistance, increased pancreatic mass, increased islet volume density with elevated alpha and beta cell mass, hypertrophied islets characterized by an altered distribution of alpha and beta cells, and weak pancreatic-duodenal homeobox (Pdx1) immunoreactivity. Conclusions: A maternal HF diet consumed during the preconception period and throughout the gestation and lactation in mice promotes metabolic and pancreatic programming in F1 and F2 male offspring, suggesting that these changes are intergenerational.