Efeitos da obesidade na migração, proliferação e diferenciação de células-tronco da medula óssea de camundongos Swiss

Abstract

Cell therapy aims to create alternatives for regeneration of injured tissues. With Discovery of diferente types of stem cells, it use gained stength by enabling repopulation beyond its modulation potential. In this case, the Mesenchymal Stem Cells (MSC) gain prominence, as they are known for their paracrine anti-inflammatory effect and absence of expression of molecules of the imune system. One of main sources of MSC is the bone marrow. However, metabolic diseases such as obesity are capable of altering the microenvironment of the bone marrow, altering the therapeutic properties of the existing cells. Therefore, the evaluation of the physiological conditions found in obese bone marrow cells is of fundamental importance, since their capacity for proliferation, migration and differentiation are importante for the success of the cellular therapy. For this study we used the modelo f hypernutition during lactation (hyperlactation) for the induction of obesity. The migration, proliferation and adipogenic and osteogenic differentiation of cells from the bone marrow were evaluated. Our results showed that obesity has systemic effects, beside decresing migratory and proliferative capacity and increasing adipogenic differentiation and reducing osteogenic differentiation. Therefore, it s possible to affirm that hypernutition during lactation induces obesity in adulthood ant that it s capable of altering characteristics crucial to the success of a stem cell transplant.