Cultura de tecidos e análise fitoquímica de Arachis repens HANDRO.

Abstract

Arachis species are known as groundnut and their seeds display high oil and protein contents, being consumed in natura. The most common commercially cultivated species of the genus is Arachis hypogaea. Other Arachis species can also be used as food, ornamental, ground cover and provide weed control. Arachis repens, commonly known as grama amendoim, is used as an ornamental, forage legume and ground cover in substitution of common grass species. Different bioactive substances have been identified in A. hypogaea. However, these substances have not been described in other species of the genus. The goal of this work was the establishment of in vitro culture for Arachis repens, aiming at the micropropagation and the comparative analysis of the resveratrol production in ethanolic extracts of the in vitro produced material, in comparison to in vivo plants. Nodal, internodal and leaf segments were excised from in vitro plants and inoculated on MS medium supplemented with different concentrations of BAP, TDZ, IAA and KIN, used alone or in combination. Explants showed distinct responses in according to the growth regulator used. In the presence of BAP, compact calluses were formed, with the production of shoots in different frequencies. In the presence of TDZ, nodal and internodal segments formed compact calluses in response to all concentrations. Nodal segments cultivated in the presence of different IAA concentrations in combination with KIN showed callogenesis The evaluation of antioxidant activity and the presence of total phenolics in extracts of different in vivo and in vitro materials was also evaluated. The HPLC analysis detected the presence of resveratrol in the materials produced in vivo and in vitro. Therefore, this work provided preliminary results about the possible use of A. repens for the production of special metabolites.