Efeito do uso de vitamina C na prevenção de lesões cerebrais por reperfusão: estudo experimental em ratos

Abstract

Reperfusion syndrome after carotid endarterectomy is a complication associated with cerebrovascular self-regulation in a chronically hypoperfused cerebral hemisphere, leading to neurological damage. Vitamin C is an important antioxidant in blood metabolism that has been shown some neuroprotective actions. This study aims to evaluate the potential effects of vitamin C in the treatment of cerebral reperfusion compared to placebo (saline) in rats. Experiments were performed with male Wistar rats divided into 3 groups: i) Sham (n = 4), animals receiving intraperitoneal injection of NaCl 0.9% saline solution (0.1 mL / kg) and underwent carotid artery dissection without clamping; ii) Control (n = 4), animals receiving intraperitoneal injection of NaCl 0.9% saline solution (0.1 mL / kg) and submitted to dissection of the carotid arteries with temporary clamping of the two arteries; iii) Vitamin C (n = 4), animals receiving intraperitoneal injection of vitamin C (750mg / kg) and underwent a carotid artery dissection with temporary clamping. Behavioral assessment was performed in all groups, including the open field, the Morris water maze test, and the Rotarod test. The levels of malondialdehyde (MDA) not hippocampus and striatum were measured using fluorometry. The results of the experiment were compared with the same response parameters of the sham group (p> 0.05), and the different types of controls (p <0.05). MDA levels in the hippocampus and striatum were also decreased in the vitamin C group compared to placebo (p <0.05). The results found allowed vitamin C to be associated with induced cerebral ischemia in rats.