Análise da região 5 não traduzida (5 utr) em sequências anotadas como trans-sialidase no genoma de Trypanosoma cruzi

Abstract

Trans-sialidase (TS) is a membrane glycoprotein belonging to a family of genes from multiple copies, involved in the process of cell invasion of the vertebrate host by the Trypanosoma cruzi (T. cruzi) parasite. This dissertation was developed with an ample analytical component that depended on the publicly available genome database or on the sequences from the T. cruzi genome project and trans-sialidase cDNAs sequences deposited in the Genbank-dbEST. This analytical component needed to be supplemented and expanded with the acquisition of new test sequences, from the methodology based on reverse transcription coupled to PCR. The fragments obtained from T. cruzi strains corresponding to the different groups were cloned, sequenced and compositionally analyzed. These sequences were edited and aligned using the CLUSTAL X software.Homologous sequences to trans-sialidase were searched against the dbEST databank. This search for similarity was performed individually with the access numbers in the sequence mentioned above against the dbEST using the BLAST to have functional and evolutionary information. Experimental methods were developed that allowed us to evaluate segments of 5 'UTR, such as the trans splicing additional or multiples sites found in the TSand their respective signals (region rich in pirimidine), compositionally variation and size of the sequences between different strains of T. cruzi. The result of this analysis showed us the amount of cDNAs related to trans-sialidase in the dbEST as well as the relationship of this cDNAs with the mini-exon.